Flavinkins (@Flavinkins)

Here is even more: remember that other pre-pandemic antibodies paper? https://journals.sagepub.com/doi/full/10.1177/0300891620974755 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8778320/ Here is the comparison of the detailed temporal distribution of the positive cases from the measles study by Amandola et. al. The SMILES cohort (the ones that found a temporal signal in antibody positivity) by Apolone and Montomoli in 2020. and the blinded re-testing of samples generated from the SMILES cohort in two independent labs in 2021. The positive SARS-CoV-2 RNA results from the "young" samples (age <20 y, given measles screening is conducted mostly on children) coincided in time with the detection of IgG antibodies from aged lung cancer patients in the initial Montomoli et. al paper, and clustered detection of antibodies within the 2022 retesting effort. This is expected as children are most likely infected by adults during the transmission of SARS-CoV-2, with or without respiratory symptoms. The positive SARS-CoV-2 RNA results from the "non-young" samples (from mid-December 2019 to early-January 2020, age >20 y as the evolving virus begins to manifest dermatological symptoms in adults) (The dermatosis sample reported by Gianotti et. al. on 10/11/2019, incidentally, also lands ~2 weeks before the detection of IgG on "Nov week 2" by Montomoli et. al.) were found to be ~2 weeks before the detection of IgG within the initial Montomoli et. al. SMILES paper. This is expected as adult infections take on average ~14 DPI to begin generating IgG. Amandola et. al (using PCR methods for detection of SARS-CoV-2 nucleic acids), Montomoli et. al (testing for SARS-CoV-2 RBD-specific antibodies), and Gianotti et. al. (testing for SARS-CoV-2 RNA and antigens using RNA-FISH and Immunohistochemistry) used entirely different (and orthogonal) technologies. None of these efforts shared even a single author with one another--yet their timeline (temporal signal and clustering of positive detections) matched each other closely. If an outbreak generates positive SARS-CoV-2 antibodies, tests positive for sequence-verified SARS-CoV-2 RNA, and yields antigens that reacted with SARS-CoV-2 on immunohistochemistry staining, across independent and orthogonal studies at the same time frame, then it is most likely an outbreak caused by some strain of SARS-CoV-2. Also see https://arkmedic.substack.com/p/the-killing-fields-of-samoa/comment/10709283