Flavinkins (@Flavinkins)

https://www.nytimes.com/2022/07/15/science/bat-coronavirus-laboratory-experiments.html BANAL-236 failed to generate a furin cleavage site when cultured in either human intestinal cells or mouse lungs that expresses human ACE2. Despite 6 passages in each, no evidence of a functional furin cleavage site can be found in the deep sequencing data, nor could any evidence of insertions be found with RT-PCR amplification of the S1-S2 boundary. This correlates well to other non-bat hosted Sarbecoviruses, such as animal or human hosted SARS-CoV, GD-CoV, GX-CoV and to an extent the CoV that formed SARS IgG antibodies inside mojiang miners. https://archive.vn/ZwOWO https://archive.vn/vUy8n As no evidence of a functional FCS have been found inside any bat-hosted Sarbecovirus samples, even with deep sequencing of the samples, with the required nucleotide arrangement (a single transversion for the bat samples in question) in the bat samples to be below the expected noise background of the reverse transcriptase used in the sequencing process, and considering that the intestinal environment such as liquids from the colon, the host environment within bats, https://pubmed.ncbi.nlm.nih.gov/35765598/ inactivates SARS-CoV-2 virions within an hour, https://archive.vn/yXjt4 https://archive.vn/Nfu5p https://archive.is/6qUW3 https://archive.vn/az10E Engineering have became the only option for the FCS to emerge in any host—just like what’s outlined in the DEFUSE documents. https://archive.vn/MafMT https://archive.vn/3r0yW https://archive.vn/jSnG9 https://archive.vn/0f06s https://web.archive.org/web/20220830161054/https://threadreaderapp.com/thread/1533688359624724486.html https://web.archive.org/web/20220830160827/https://twitter.com/kevin_mckernan/status/1533773213326528512?s=21&t=yfKZbY_ZkCOAl5StQOTF7A “And their sequencing data doesn’t say anything other than how incompetent or deceptive they are. 90 cycles of PCR across an Region notorious for Illumina sequencing error is farcical. ” @Kevin_McKernan What’s more interesting is that evidence of engineering can be seen in SARS-CoV-2 especially when compared to the closest relatives, RaTG13, ZC45 and BANALCoVs. All of which contained two BsaI sites within the F3 fragment of the ORF1a, highly conserved in Asian SARS-like Coronaviruses. That interfered with the F3 fragment in both of the available SARS-CoV-2 reverse genetic systems that is uniquely missing inside SARS-CoV-2. https://archive.vn/CoMp6 https://archive.vn/Ast3x https://archive.vn/K7qsT