Flavinkins (@Flavinkins)

“In his inaugural research post, @jhas5 presents his theory for how a Duke-NUS bat researcher inadvertently might have become patient zero while testing a self-spreading bat vax at Wuhan's BSL-4 lab (her "office" in 2019). Thoughts on his hypothesis? ” @Capitolsheila https://jimhaslam.substack.com/p/1-a-consensus-sequence-of-sars-like Some background: https://archive.ph/CFNcS https://archive.ph/VdpwJ https://archive.ph/NFgcB Why a FCS? The S1-S2 FCS is both necessary for efficient transmission through the respiratory route in most animals, and disables the ability for a Sarbecovirus to form reservoirs within said animals by inactivating intestinal shedding of the virus and preventing the proper reinfection of animal hosts (or even human hosts before the roll-out of Adenovirus-based vaccines https://www.medrxiv.org/content/10.1101/2020.08.24.20179457v2). This makes such a virus a perfect candidate for self-spreading vaccines as it is both spread efficiently (for the animal models they tested in North America) and disappears from the target population once the population have been fully immunized. There is a reason why no other Sarbecoviruses ever found in bats or in any other animals contained a FCS. https://archive.ph/dorVk https://archive.ph/qXMsd https://archive.ph/71Di3 https://archive.ph/eiWvH https://www.medrxiv.org/content/10.1101/2020.08.24.20179457v2 https://www.medrxiv.org/content/10.1101/2020.10.14.20212720v1.full-text https://www.researchgate.net/profile/Christian-Hansen-12/publication/350138532_Assessment_of_protection_against_reinfection_with_SARS-CoV-2_among_4_million_PCR-tested_individuals_in_Denmark_in_2020_a_population-level_observational_study/links/605b14bca6fdccbfea00b5d3/Assessment-of-protection-against-reinfection-with-SARS-CoV-2-among-4-million-PCR-tested-individuals-in-Denmark-in-2020-a-population-level-observational-study.pdf?origin=publication_detail https://gab.com/Flavinkins/posts/108677210789525953 Why PRRARS? P681 is necessary for the efficient production of viral stocks within VERO E6 cells and PRRARS was the most lung- and airway- compatible FCS that the VERO E6 cell line will still accept and grow efficiently. Passage of the virus within airway cell line models while preparing the stock in VERO E6 turn an otherwise canonical RRRARRS sequence into PRRARS. (So will VERO passage with frequent plaque purification and western blotting that ensure the Spike protein produced is still cleaved)