Flavinkins (@Flavinkins)

https://archive.vn/LmQEf “If an insertion was placed adjacent to the synonymous mutation, it would've appeared to be an out-of-frame” Remember that regardless of VERO/HAE or CaLu-3, The best S1-S2 peptide in a cell culture is always PRRARS—and nothing else. Both RRRARR and ARRAR turns into PRRAR upon culturing within these 2 cell models. https://journals.asm.org/doi/10.1128/JVI.00074-08 Reason? VERO and CaLu-3 cell lines, but especially VERO cell line, glycosylates the S1-S2 if there is a P681. The allow the RRAR to stay uncleaved, and subsequently become available to bind to the HSPG (heparan sulfate proteoglycans) on the surface of these cells. This improves in-vitro infectivity and growth of the virus at the expense of in-vivo transmissibility. This is also why P681 is lost during the in-vivo circulation of SARS-CoV-2 in current VOCs. https://archive.vn/3r0yW https://archive.vn/Zlss7 https://archive.vn/6B1Og https://archive.vn/oER1M The high immunogenicity of the FCS https://archive.vn/Nfu5p https://archive.is/6qUW3 can be either a safety feature or an intended feature for a self-spreading vaccine in wild animals—we know that all CoVs in wild animal reservoirs are enteric. What you will do if you need to create a CoV strain that is both effective at spreading in your target population yet have the lowest chance of remaining in the reservoir once it have performed the intended function of immunizing said population? (Hint: which organ is known to confer high transmissibility experimentally yet not used by animal viruses in the wild?) https://archive.vn/VdpwJ https://archive.md/qXMsd https://archive.vn/az10E https://archive.vn/dorVk https://archive.vn/CFNcS https://archive.vn/NFgcB https://gab.com/Flavinkins/posts/108779333080481680 https://archive.vn/Fdtgo (Other signs of vaccine development within the SARS-CoV-2 genome) https://gab.com/Flavinkins/posts/108709042074566995 https://archive.ph/Ohupc https://archive.ph/QanYk https://archive.ph/TrTW5 https://archive.md/71Di3 https://archive.md/eiWvH